Populations with higher than the overall average percentage of males are colored orange while those populations with a lower than average male percentage are colored blue. As we have not found any sign for CSD in the species, we propose that the zebrafish has a polygenic sex determination system.
As we expected that a sex-determining chromosomal region would be present in all strains, we analyzed the five common CNVRs on individuals, but none of them turned out to be inherited in a sex-linked pattern Fig. Genet Res SNP-based linkage map for zebrafish reveals sex determination loci.
Experientia — View Article Google Scholar 4. For each experiment the overall sex ratios varied, but both showed about a twenty percent increase in male percentage in populations with starting densities of fish per 1. Red boxes indicate the sex-linked markers that were confirmed through individual testing.
Grunwald D, Eisen J Headwaters of the zebrafish emergence of a new model vertebrate. Moreover, most of the current knowledge on zebrafish sexual development is related to its gonad differentiation for reviews see  while the mode of its sex determination is still disputed.
Figure S3 Sex-linked FluoMEP markers obtained by Published data and our results both seem to suggest that non- bulk segregant analysis performed on pooled male and extreme environmental factors do not have a major effect on female samples of guppy Poecilia reticulata.
Temperature is the most commonly studied environmental cue for sex determination. Although it has higher resolution than our CNV array the study did not performed comparative analysis of the male and female genome. Multifactorial selective breeding was carried out over a few generations for five lines of zebrafish to select for pairs that produced a sex-biased family.
Individuals with different families; ii parental genotypes have a major effect on the intermediate body shape and poorly observable papilla were sex ratio; and iii one of the two sexes can be depleted through gently squeezed and checked for eggs or sperm. References 1. We have tested the effect of rearing density on zebrafish sex, and found a substantial Figure S1 Multifactorial selective breeding was carried increase of males at high density individuals per 1.
Table 2. As our breeding data also do not support the show that zebrafish uses primarily genetic sex determination presence of a sex chromosome, we provide a strong case against system. The high R 2 value indicates that sex ratios between 1st and 2nd crosses from the same breeding pair are very similar.
Published data and our results both seem to suggest that non-extreme environmental factors do not have a major effect on zebrafish sex ratio.